A heart from a flap of skin: developing a machine that will repair any organ or tissue from the cells of our skin. A concrete hope for regenerative medicine and treatment of serious diseases, otherwise intractable
A veritable "time machine" able to bring stem cells rather than ancestral State, embryonic-like, to help molecular biologists to "reprogramme" and turn them into any cell type. It's called REAC (Radio Electric Asymmetric Conveyer)-configured as Bio-Neuro Enhancer Enhancer (B.E.N.E.)-and it is a conveyor that produces radio fields at very low intensity.
For the first time in the literature the reprogramming of adult cells was obtained through the use of a low-emission intensity of a spectral field and not with the use of genetic engineering or with the use of viral vectors as already experienced by Prof. Shinya Yamanaka, neo-Nobel Prize for medicine.
The study and the research findings of the team of researchers led by Carlo Ventura, a Professor of Molecular Biology at the University of Bologna ("Radio Electric fields directly Conveyed to reprogram human skin Fibroblast dermal toward Cardiac, Neuronal, and Skeletal Muscle-like lineages")-published in Cell Transplantation, the prestigious American Journal of regenerative medicine-open up unprecedented prospects. The discovery is the result of the synergy between the laboratory of molecular biology and stem cell Bioengineering, directed by Prof. With the collaboration of Ventura Dr.ssa Claudia Casey (cardio-vascular Department of the University of Bologna), the Institute of Florence with Fountains Rinaldi, his research team as part of regenerative medicine, coordinated by Prof. Salvatore Rinaldi and constituted by Dr.ssa Vania Fountains and Dr. Alexander Carpenter and the Department of Biomedical Sciences of the University of Sassari, with a research team coordinated by Dr.ssa Margaret Maioli and constituted by Dr.ssa Sara Santaniello, by Dr. Gianfranco Pigliaru and Dr.ssa Sara Gualini.
"The fibroblasts represent the essential cellular component of connective tissue which in turn constitutes a major part of every organ and tissue," says prof. Ventura. "It's so precisely by fibroblasts which could go through the process of reprogramming just discovered, an absolutely general mechanism of repair and regeneration for organs and tissues damaged by various diseases".
Stem cells offer an increasingly concrete hope for cure and healing in serious diseases, often fatal or high impact on quality of life, currently not treatable even with the most advanced pharmacological remedies and/or surgery.
Human adult stem cells can be obtained from various sources, including adipose tissue: at the time of the levy are in a State in which (undifferentiated) still do not look like any of an individual's adult cells. Under certain physical and chemical stimuli, these cells have the ability to transform (differentiate) in vitro and in vivo in different cell types that make up the different organs and apparatus. The percentage of adult stem cells that take on these new roads, with success, is very low.
"The kind of reprogramming obtained for the first time since research team -explains Margaret dott.ssa Maioli-is a direct cell reprogramming. Instead of sending a non-adult stem cell back in time until it becomes almost embryonic and then from there to get a differentiation has managed to turn this mature cell a direct route, or to direct it towards more cell fates, in this case, cardiac, and skeletal muscle, neuronal, as if you departed already from a embryonic ".
Another novelty was to avoid the use of viral vectors and genetic engineering techniques. REAC technology ", explains Prof. Rinaldi-and in particular the specific protocols developed specifically for this study lead safely, without risk of cancer, changes in cell fate related to the modulation of gene expression and molecular signals network and physicists that control cellular homeostasis. For the first time in the literature the reprogramming of adult cells, in this case fibroblasts, was obtained thanks to the asymmetric routing within a cell culture of Radioelectric field emission at very low intensity, produced by B.E.N.E. and exceptional equipment also as human fibroblasts rescheduled by the research team showed the same efficiency that would transform from embryonic stem cellsas this research team had already demonstrated. In fact, the percentages obtained differentiation with REAC technology have vastly superior to any other previous technique, producing cell lines immediately usable and safe.
Concludes prof. Ventura: "our study used therapeutically immediately makes the reprogramming of fibroblasts, representing the evolution of the work started by Prof. Shinya Yamanaka, neo-Nobel Prize in medicine ".
Translated by Google Translator